Article
In-cell polymerase chain reaction: strategy and diagnostic applications.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2006
Hviid T Vauvert
Abstract excerpt
In situ polymerase chain reaction (PCR) refers to the amplification of specific nucleic acid sequences and subsequent visualization of the PCR products in tissue sections. When PCR is performed in fixed cells in suspension the term in-cell PCR is normally applied instead. For example, in-cell PCR has been used for intracellular detection of specific viruses. Furthermore, in mixtures of cells in suspension with...
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