Integrated cytochrome P450 reaction phenotyping: attempting to bridge the gap between cDNA-expressed cytochromes P450 and native human liver microsomes.

doi:10.1016/s0006-2952(98)00268-8

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Article

Integrated cytochrome P450 reaction phenotyping: attempting to bridge the gap between cDNA-expressed cytochromes P450 and native human liver microsomes.

Biochemical pharmacology - 1 Mar 1999

Rodrigues A D

Abstract excerpt

With the increased availability of human liver tissue, recombinant (cDNA-expressed) cytochrome P450 proteins (rCYPs), and knowledge of the human CYP pool (e.g. immunoquantitated levels of each CYP form in native liver microsomes), it is now possible to carry out in vitro "CYP reaction phenotyping...

Topics

Cytochrome P-450 Enzyme System
DNA, Complementary
Enzyme Inhibitors
Humans
Isoenzymes
Kinetics
Microsomes, Liver
Phenotype
Recombinant Proteins

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