A framework for optimized reaction phenotyping of the seven major hepatic P450 isoforms in human liver microsomes using six mechanism-based inactivators and one reversible inhibitor.

Abstract excerpt

Cytochrome P450 (CYP) enzymes play a central role in drug metabolism. Understanding the contribution of individual CYP isoforms, known as CYP reaction phenotyping, is essential for elucidating metabolic pathways and predicting drug-drug interactions. Reversible inhibitors are commonly used in human liver microsome (HLM)-based CYP reaction phenotyping. However, selecting appropriate inhibitor concentrations is...

Topics

Join the communities discussing this publication.

• Cytochrome P-450 Enzyme Inhibitors
• Cytochrome P-450 Enzyme System
• Drug Interactions
• Isoenzymes
• Microsomes, Liver
• Phenotype