Article
Reactions of Cre with methylphosphonate DNA: similarities and contrasts with Flp and vaccinia topoisomerase.
PloS one - 30 Sept 2009
Ma Chien-Hui, Kachroo Aashiq H, Macieszak Anna, Chen Tzu-Yang, Guga Piotr, Jayaram Makkuni
Abstract excerpt
BACKGROUND: Reactions of vaccinia topoisomerase and the tyrosine site-specific recombinase Flp with methylphosphonate (MeP) substituted DNA substrates, have provided important insights into the electrostatic features of the strand cleavage and strand joining steps catalyzed by them. A conserved arginine residue in the catalytic pentad, Arg-223 in topoisomerase and Arg-308 in Flp, is not essential for stabilizing...
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