Article
Identification of the ribosome binding sites of translation initiation factor IF3 by multidimensional heteronuclear NMR spectroscopy.
RNA (New York, N.Y.) - 1 Jan 1999
Sette M, Spurio R, van Tilborg P, Gualerzi C O, Boelens R
Abstract excerpt
Titrations of Escherichia coli translation initiation factor IF3, isotopically labeled with 15N, with 30S ribosomal subunits were followed by NMR by recording two-dimensional (15N,1H)-HSQC spectra. In the titrations, intensity changes are observed for cross peaks belonging to amides of individual amino acids. At low concentrations of ribosomal subunits, only resonances belonging to amino acids of the C-domain of...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.
