Article
Identification of the ribosome binding sites of translation initiation factor IF3 by multidimensional heteronuclear NMR spectroscopy.
RNA (New York, N.Y.) - 1 Jan 1999
Sette M, Spurio R, van Tilborg P, Gualerzi C O, Boelens R
Abstract excerpt
Titrations of Escherichia coli translation initiation factor IF3, isotopically labeled with 15N, with 30S ribosomal subunits were followed by NMR by recording two-dimensional (15N,1H)-HSQC spectra. In the titrations, intensity changes are observed for cross peaks belonging to amides of individual...
Topics
- Amino Acid Sequence
- Bacterial Proteins
- Binding Sites
- Escherichia coli
- Eukaryotic Initiation Factor-3
- Magnetic Resonance Spectroscopy
- Models, Molecular
- Molecular Sequence Data
- Mutation
- Peptide Initiation Factors
- Protein Conformation