Article
Sequence-specific 1H-NMR assignment and secondary structure of the Tyr41----His mutant of the single-stranded DNA binding protein, gene V protein, encoded by the filamentous bacteriophage M13.
European journal of biochemistry - 5 Dec 1991
Folkers P J, van Duynhoven J P, Jonker A J, Harmsen B J, Konings R N, Hilbers C W
Abstract excerpt
Sequence-specific 1H-NMR assignments are reported for the Tyr41----His (Y41H) mutant of the single-stranded DNA binding protein, encoded by gene V of the filamentous bacteriophage M13 (GVP). The mutant protein was chosen for this purpose because it exhibits significantly improved solubility characteristics over wild-type GVP [Folkers et al. (1991) Eur. J. Biochem. 200, 139-148]. The secondary structure elements...
Topics
- Amino Acid Sequence
- Bacteriophages
- DNA-Binding Proteins
- Genes, Viral
- Histidine
- Magnetic Resonance Spectroscopy
- Molecular Sequence Data
- Mutation
- Protein Conformation
- Tyrosine