Article
Regulation of the spatial organization of mesenchymal connective tissue: effects of cell-associated versus released isoforms of platelet-derived growth factor.
The American journal of pathology - 1 Jan 1999
Eming S A, Yarmush M L, Krueger G G, Morgan J R
Abstract excerpt
Platelet-derived growth factor (PDGF), a mitogen and chemoattractant for mesenchymal cells, occurs as cell-associated or released isoforms. To investigate their in vivo role, human keratinocytes, which normally synthesize both types of PDGF, were genetically modified to overexpress either wild-type PDGF-B (cell-associated) or the truncation mutant PDGF-B211 (released). Cells expressing the mutant isoform released...
Topics
- Animals
- Basement Membrane
- Becaplermin
- Cells, Cultured
- Connective Tissue
- Gene Transfer Techniques
- Humans
- Isomerism
- Keratinocytes
- Mesoderm
- Mice
- Mice, Nude
- Mutation
- Platelet-Derived Growth Factor
- Proto-Oncogene Proteins c-sis