Article
Abnormal collagen assembly, though normal phenotype, in alginate bead cultures of chick embryo chondrocytes.
Experimental cell research - 10 Jan 1999
Gregory K E, Marsden M E, Anderson-MacKenzie J, Bard J B, Bruckner P, Farjanel J, Robins S P, Hulmes D J
Abstract excerpt
The collagens produced by chick embryo chondrocytes cultured in alginate beads were investigated both biochemically and ultrastructurally. The cartilage phenotype is maintained for at least 14 days, as indicated by the production of the cartilage-specific collagens II, IX, and XI and the absence...
Topics
- Alginates
- Aminopropionitrile
- Animals
- Cartilage, Articular
- Cell Culture Techniques
- Cells, Cultured
- Chick Embryo
- Chondrocytes
- Chondroitin ABC Lyase
- Chromatography
- Collagen
- Crystallization
- Culture Media, Conditioned
- Extracellular Matrix
- Fluorometry
- Hydroxyproline
- Microscopy, Electron
- Microspheres