Article
Phenotypic stability of bovine articular chondrocytes after long-term culture in alginate beads.
Journal of cell science - 1 Jan 1994
Häuselmann H J, Fernandes R J, Mok S S, Schmid T M, Block J A, Aydelotte M B, Kuettner K E, Thonar E J
Abstract excerpt
Articular chondrocytes embedded in alginate gel produce de novo a matrix rich in collagens and proteoglycans. A major advantage of this culture system is that the cells can be recovered by chelating the calcium, which otherwise maintains the alginate in its gel state. Chondrocytes thus released a...
Topics
- Alginates
- Animals
- Cartilage, Articular
- Cattle
- Cells, Cultured
- Chromatography, Ion Exchange
- Collagen
- Culture Techniques
- Gels
- Glucuronic Acid
- Hexuronic Acids
- Male
- Microscopy, Electron
- Orchiectomy
- Phenotype
- Proline
- Proteoglycans
- Sulfates