Contribution of Taq polymerase-induced errors to the estimation of RNA virus diversity.

doi:10.1099/0022-1317-79-12-2921

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Article

Contribution of Taq polymerase-induced errors to the estimation of RNA virus diversity.

The Journal of general virology - 1 Dec 1998

Bracho M A, Moya A, Barrio E

Abstract excerpt

The genetic diversity of a vesicular stomatitis virus population was analysed by RT-PCR, cloning and sequencing of two approximately 500 nucleotide regions of the virus genome. PCR amplifications were performed in parallel experiments with both Taq and Pfu DNA polymerases, and important differenc...

Topics

Animals
Cell Line
Cricetinae
Genetic Variation
Membrane Glycoproteins
Mutation
Phosphoproteins
Polymerase Chain Reaction
Reproducibility of Results
Taq Polymerase
Vesicular stomatitis Indiana virus
Viral Envelope Proteins
Viral Structural Proteins

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