Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

From RNA to quasispecies: a DNA polymerase with proofreading activity is highly recommended for accurate assessment of viral diversity.

Journal of virological methods - 1 May 2003

Malet Isabelle, Belnard Magali, Agut Henri, Cahour Annie

Abstract excerpt

RNA viruses are characterized by their high rates of genetic variation. Their genetic diversity is generally studied by reverse transcription (RT) followed by polymerase chain reaction (PCR) amplification and nucleotide (nt) sequence determination. The misinterpretation of viral diversity due to copy errors introduced by the enzymes used in this two-step protocol has not yet been assessed systematically. In order...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to 5' Untranslated Regions
Share to DNA-Directed DNA Polymerase

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Genetic Variation

Share to Hepacivirus

Share to Mutation

Share to Reverse Transcriptase Polymerase Chain Reaction

Share to RNA Viruses

Share to Transcription, Genetic

Source

Identifiers

PMID: 12711059
DOI: 10.1016/s0166-0934(03)00067-3

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

From RNA to quasispecies: a DNA polymerase with proofreading activity is highly recommended for accurate assessment of viral diversity.PMID 12711059Read ↗

Inferred hepatitis C virus quasispecies diversity is influenced by choice of DNA polymerase in reverse transcriptase-polymerase chain reactions.Semantic proximity Read ↗

HCV genetic variability: from quasispecies evolution to genotype classification.Semantic proximity Read ↗

Genetic variability: the key problem in the prevention and therapy of RNA-based virus infections.Semantic proximity Read ↗

Hepatitis C virus quasispecies in cancerous and noncancerous hepatic lesions: the core protein-encoding region.Semantic proximity Read ↗

Coronaviruses: An RNA proofreading machine regulates replication fidelity and diversitySemantic proximity Read ↗

Coronaviruses: an RNA proofreading machine regulates replication fidelity and diversity.Semantic proximity Read ↗

Fidelity Variants and RNA Quasispecies.Semantic proximity Read ↗

Comparison of amplification enzymes for Hepatitis C Virus quasispecies analysis.Semantic proximity Read ↗

Mutation Rates, Mutation Frequencies, and Proofreading-Repair Activities in RNA Virus Genetics.Semantic proximity Read ↗

The three faces of riboviral spontaneous mutation: spectrum, mode of genome replication, and mutation rate.Semantic proximity Read ↗

Accurate representation of the hepatitis C virus quasispecies in 5.2-kilobase amplicons.Semantic proximity Read ↗

Do Taq-generated RT-PCR products from RNA viruses accurately reflect viral genetic heterogeneity?Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.