Article
Use of dsRNA-mediated genetic interference to demonstrate that frizzled and frizzled 2 act in the wingless pathway.
Cell - 23 Dec 1998
Kennerdell J R, Carthew R W
Abstract excerpt
We investigated the potential of double-stranded RNA to interfere with the function of genes in Drosophila. Injection of dsRNA into embryos resulted in potent and specific interference of several genes that were tested. In contrast, single-stranded RNA weakly interfered with gene activity. The me...
Topics
- Animals
- Bacterial Proteins
- Body Patterning
- Drosophila Proteins
- Drosophila melanogaster
- Epistasis, Genetic
- Frizzled Receptors
- Fushi Tarazu Transcription Factors
- Glycogen Synthase Kinase 3
- Homeodomain Proteins
- Immunohistochemistry
- Injections
- Insect Proteins
- Membrane Proteins
- Nucleic Acid Hybridization
- Phenotype
- Protein Serine-Threonine Kinases
- Proto-Oncogene Proteins