Article
Silencing of Toll pathway components by direct injection of double-stranded RNA into Drosophila adult flies.
Nucleic acids research - 15 Nov 2003
Goto Akira, Blandin Stéphanie, Royet Julien, Reichhart Jean-Marc, Levashina Elena A
Abstract excerpt
Double-stranded RNA (dsRNA) gene interference is an efficient method to silence gene expression in a sequence-specific manner. Here we show that the direct injection of dsRNA can be used in adult Drosophila flies to disrupt function of endogenous genes in vivo. As a proof of principle, we have used this method to silence components of a major signaling cascade, the Toll pathway, which controls fruit fly...
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