Article
[Establishment of mutant strain from human lung cancer cell (A549) with characteristics of resistance to 8-AG and HGPRT deficiency].
Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University - 1 Jan 1997
Xiao Y, Hu C, Shi K, Luo M
Abstract excerpt
A human lung cancer cell line (A549) mutant with hypoxanthine guanine phosphoribosyltransferase (HGPRT) deficiency and resistance to 8-AG was established by treatment of the A549 cells with 3 micrograms.ml-1 N-methyl-N'-nitro-nitrosoguanidine (MNNG), and prescreened in 0.25% agarose DMEM semisoli...
Topics
- Antimetabolites, Antineoplastic
- Azaguanine
- Cell Fusion
- Cell Line, Transformed
- Drug Resistance, Neoplasm
- Humans
- Hypoxanthine Phosphoribosyltransferase
- Lung Neoplasms
- Methylnitronitrosoguanidine
- Mutation
- Tumor Cells, Cultured