Article
[Effect of mouse p53 minigene on lung cancer cells with different 172 structures regulated by tetracycline].
Zhonghua bing li xue za zhi = Chinese journal of pathology - 1 Oct 2000
Ma Y, Wu B, Xie J, You J, Liu J, Cui X, Wang J, Hui P
Abstract excerpt
OBJECTIVE: To investigate the effect of mouse 172 wild-type p53 (Arg), pseudo-wild-mutant-type p53 (Arg-->Leu) and mutant-type p53 (Arg-->His) induced by absence of tetracycline on the growth of PG cell line. METHODS: Three variant types of p53 minigene were sub-cloned by gene recombination into an expression vector which was controlled by tetracycline. Through LipofectAMINE, the vectors were transfected into p53...
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