Article
Natural agonist enhancing bis-His zinc-site in transmembrane segment V of the tachykinin NK3 receptor.
FEBS letters - 13 Nov 1998
Rosenkilde M M, Lucibello M, Holst B, Schwartz T W
Abstract excerpt
In the wild-type tachykinin NK3A receptor histidyl residues are present at two positions in TM-V, V:01 and V:05, at which Zn2+ functions as an antagonist in NK1 and kappa-opioid receptors with engineered metal-ion sites. Surprisingly, in the NK3A receptor Zn2+ instead increased the binding of the agonist 125I-[MePhe7]neurokinin B to 150%. [MePhe7]neurokinin B bound to the NK3A receptor in a two-component mode of...
Topics
- Amino Acid Sequence
- Animals
- Binding, Competitive
- COS Cells
- Histidine
- Humans
- Membrane Proteins
- Molecular Sequence Data
- Mutation
- Neurokinin B
- Protein Conformation