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Article

C-terminal deletion mutants of the FokI restriction endonuclease.

Gene - 29 Oct 1993

Li L, Wu L P, Clarke R, Chandrasegaran S

Abstract excerpt

We have constructed two C-terminal deletion mutants of the FokI restriction endonuclease by using the polymerase-chain-reaction technique and expressed them in Escherichia coli. The two mutant proteins (MP) of 41 and 30 kDa, were purified to homogeneity and their DNA-binding properties were characterized. The 41-kDa MP specifically binds the DNA sequence, 5'-GGATG/3'-CCTAC, like the wild-type (wt) FokI, but does...

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PMID: 8224897
DOI: 10.1016/0378-1119(93)90227-t

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