Article
C-terminal deletion mutants of the FokI restriction endonuclease.
Gene - 29 Oct 1993
Li L, Wu L P, Clarke R, Chandrasegaran S
Abstract excerpt
We have constructed two C-terminal deletion mutants of the FokI restriction endonuclease by using the polymerase-chain-reaction technique and expressed them in Escherichia coli. The two mutant proteins (MP) of 41 and 30 kDa, were purified to homogeneity and their DNA-binding properties were characterized. The 41-kDa MP specifically binds the DNA sequence, 5'-GGATG/3'-CCTAC, like the wild-type (wt) FokI, but does...
Topics
- Amino Acid Sequence
- Base Sequence
- Cloning, Molecular
- DNA, Bacterial
- Deoxyribonucleases, Type II Site-Specific
- Electrophoresis, Polyacrylamide Gel
- Escherichia coli
- Gene Deletion
- Molecular Sequence Data
- Mutation
- Polymerase Chain Reaction