Article
C-terminal peptide of fusarium heterosporum lipase is necessary for its increasing thermostability.
Journal of biochemistry - 1 Dec 1998
Nagao T, Shimada Y, Sugihara A, Tominaga Y
Abstract excerpt
Saccharomyces cerevisiae bearing a lipase cDNA from Fusarium heterosporum produced two lipases, A and B. Lipase B was significantly more stable to temperature than lipase A, but their optimum temperatures were similar. Lipase B was composed of one polypeptide (301 amino acids), and lipase A was c...
Topics
- Amino Acid Sequence
- Blotting, Northern
- Enzyme Stability
- Fungal Proteins
- Fusarium
- Hot Temperature
- Lipase
- Molecular Sequence Data
- Molecular Weight
- Mutation
- Peptide Fragments
- RNA, Messenger
- Recombinant Proteins
- Saccharomyces cerevisiae
- Solvents
- Structure-Activity Relationship