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Article

A novel extracellular esterase from Bacillus subtilis and its conversion to a monoacylglycerol hydrolase.

European journal of biochemistry - 1 Nov 2000

Eggert T, Pencreac'h G, Douchet I, Verger R, Jaeger K E

Abstract excerpt

A novel gene lipB, which encodes an extracellular lipolytic enzyme, was identified in the Bacillus subtilis genomic DNA sequence. We have cloned and overexpressed lipB in B. subtilis and Escherichia coli and have also purified the enzyme from a B. subtilis culture supernatant to electrophoretic homogeneity. Four different lipase assays were used to determine its catalytic activity: pH-stat, spectrophotometry,...

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Identifiers

PMID: 11029590
DOI: 10.1046/j.1432-1327.2000.01736.x

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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