Article
Stable linker peptides for a cellulose-binding domain-lipase fusion protein expressed in Pichia pastoris.
Protein engineering - 1 Sept 2001
Gustavsson M, Lehtiö J, Denman S, Teeri T T, Hult K, Martinelle M
Abstract excerpt
Fusion proteins composed of a cellulose-binding domain from Neocallimastix patriciarum cellulase A and Candida antarctica lipase B were constructed using different linker peptides. The aim was to create proteolytically stable linkers that were able to join the functional modules without disrupting their function. Six fusion variants containing linkers of 4-44 residues were expressed in Pichia pastoris and...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.
