Article
Importance of the Ca2+-binding sites in the N-catalytic domain of a family I.3 lipase for activity and stability.
Protein engineering, design & selection : PEDS - 1 Dec 2008
Kuwahara K, Angkawidjaja C, Matsumura H, Koga Y, Takano K, Kanaya S
Abstract excerpt
A family I.3 lipase from Pseudomonas sp. MIS38 (PML) contains three Ca(2+)-binding sites (Ca1-Ca3) in the N-catalytic domain. Of them, the Ca1 site is formed only in an open conformation. To analyze the role of these Ca(2+)-binding sites, three mutant proteins D157A-PML, D275A-PML and D337A-PML, which are designed to remove the Ca1, Ca2 and Ca3 sites, respectively, were constructed. Of them, the crystal...
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