Article
Prokaryotic expression of bovine lactoferrin deletion mutants that bind to the Ca2+-dependent lactoferrin receptor on isolated rat hepatocytes.
Protein expression and purification - 1 Nov 1998
Sitaram M P, Moloney B, McAbee D D
Abstract excerpt
We generated a series of recombinant variants of bovine lactoferrin (Lf) as fusion proteins using two prokaryotic expression vectors and examined the ability of the expressed proteins to compete with native Lf for binding to the Ca2+-dependent Lf receptor on isolated rat hepatocytes. A near-full-...
Topics
- Animals
- Binding, Competitive
- Calcium
- Cattle
- Cells, Cultured
- Escherichia coli
- Gene Expression
- Genetic Vectors
- Lactoferrin
- Liver
- Mutation
- Rats
- Receptors, Cell Surface
- Recombinant Fusion Proteins
- Sequence Deletion
- Thioredoxins