Article
Production of recombinant bovine lactoferrin N-lobe in insect cells and its antimicrobial activity.
Protein expression and purification - 1 Apr 2001
Nakamura I, Watanabe A, Tsunemitsu H, Lee N Y, Kumura H, Shimazaki K I, Yagi Y
Abstract excerpt
Lactoferrin is a multifunctional, iron-binding glycoprotein found in physiological fluids of mammals. In the present study, a gene encoding the N-terminal half (N-lobe) of bovine lactoferrin was cloned and expressed in cultured insect cells using a baculovirus expression system. One mutation was found in the lactoferrin N-lobe gene, but it resulted in no amino acid substitution. The recombinant lactoferrin N-lobe...
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