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Article

Ligand binding domain of granulocyte colony-stimulating factor receptor.

The Journal of biological chemistry - 2 Sept 1994

Hiraoka O, Anaguchi H, Yamasaki K, Fukunaga R, Nagata S, Ota Y

Abstract excerpt

The amino-terminal domain of the cytokine receptor homologous region (BN domain; roughly 100 amino acid residues) in the receptor for murine granulocyte colony-stimulating factor (G-CSF) was secreted as a maltose-binding protein fusion into the Escherichia coli periplasm. The murine BN domain (mBN) was prepared from the fusion protein by restriction protease Factor Xa digestion and purified to homogeneity. The...

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PMID: 7520915

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