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Article

Production of soluble single-chain T-cell receptor fragments in Escherichia coli trxB mutants.

Molecular immunology - 1 Feb 1998

Molloy P E, Harris W J, Strachan G, Watts C, Cunningham C

Abstract excerpt

Antibodies and T cell receptors (TCR) both belong to the immunoglobulin superfamily whose members are characterised by the possession of one or more immunoglobulin domains. The production of soluble single chain antibody fragments in Escherichia coli has, in recent years, become a routine laboratory procedure. In contrast, the production of T cell receptors in bacteria has remained problematic as the majority of...

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PMID: 9683253
DOI: 10.1016/s0161-5890(98)00019-4

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