Article
Expression of cloned human lactoferrin in baby-hamster kidney cells.
The Biochemical journal - 1 Jun 1991
Stowell K M, Rado T A, Funk W D, Tweedie J W
Abstract excerpt
Human lactoferrin was expressed from a cloned cDNA introduced into mammalian cells in tissue culture. Total RNA was extracted from human bone marrow, and lactoferrin cDNA was synthesized by primer-specific polymerase chain reaction after oligo(dT)-primed first-strand synthesis. The cDNA was sequenced to confirm its identity with previously published human lactoferrin sequences and cloned into the eukaryotic...
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