Article
Expression of cloned human lactoferrin in baby-hamster kidney cells.
The Biochemical journal - 1 Jun 1991
Stowell K M, Rado T A, Funk W D, Tweedie J W
Abstract excerpt
Human lactoferrin was expressed from a cloned cDNA introduced into mammalian cells in tissue culture. Total RNA was extracted from human bone marrow, and lactoferrin cDNA was synthesized by primer-specific polymerase chain reaction after oligo(dT)-primed first-strand synthesis. The cDNA was seque...
Topics
- Amino Acid Sequence
- Animals
- Bone Marrow
- Cell Line
- Cloning, Molecular
- Cricetinae
- DNA
- Genetic Variation
- Humans
- Kidney
- Kinetics
- Lactoferrin
- Molecular Sequence Data
- Molecular Weight
- Monocytes
- Polymerase Chain Reaction
- Recombinant Proteins
- Transfection