Cloning, phenotypic expression, and DNA sequence of the gene for lactacin F, an antimicrobial peptide produced by Lactobacillus spp.

doi:10.1128/jb.173.5.1779-1788.1991

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Article

Cloning, phenotypic expression, and DNA sequence of the gene for lactacin F, an antimicrobial peptide produced by Lactobacillus spp.

Journal of bacteriology - 1 Mar 1991

Muriana P M, Klaenhammer T R

Abstract excerpt

Lactacin F is a heat-stable bacteriocin produced by Lactobacillus acidophilus 11088. A 63-mer oligonucleotide probe deduced from the N-terminal lactacin F amino acid sequence was used to clone the putative laf structural gene from plasmid DNA of a lactacin F-producing transconjugant, L. acidophilus T143. One clone, NCK360, harbored a recombinant plasmid, pTRK160, which contained a 2.2-kb EcoRI fragment of the...

Topics

Amino Acid Sequence
Bacteriocins
Base Sequence
Cloning, Molecular
Conjugation, Genetic
DNA, Bacterial
Escherichia coli
Genes, Bacterial
Lactobacillus
Lactobacillus acidophilus
Molecular Sequence Data

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Molecular Weight

Open Reading Frames

Phenotype

Plasmids

Promoter Regions, Genetic

Restriction Mapping

Source

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Identifiers

PMID: 1900281
DOI: 10.1128/jb.173.5.1779-1788.1991
PMCID: PMC207330

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1900281-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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