Article
Drug resistance to 5-aza-2'-deoxycytidine, 2',2'-difluorodeoxycytidine, and cytosine arabinoside conferred by retroviral-mediated transfer of human cytidine deaminase cDNA into murine cells.
Cancer chemotherapy and pharmacology - 1 Jan 1998
Eliopoulos N, Cournoyer D, Momparler R L
Abstract excerpt
PURPOSE: The hematopoietic toxicity produced by the cytosine nucleoside analogs is a critical problem that limits their effectiveness in cancer therapy. One strategy to prevent this dose-limiting toxicity would be to insert a gene for drug resistance to these analogs into normal bone marrow cells. Cytidine (CR) deaminase can deaminate and thus inactivate 5-aza-2'-deoxycytidine (5-AZA-CdR),...
Topics
- 3T3 Cells
- Animals
- Antimetabolites
- Antimetabolites, Antineoplastic
- Azacitidine
- Colony-Forming Units Assay
- Cytarabine
- Cytidine Deaminase
- DNA, Complementary
- Decitabine
- Deoxycytidine
- Drug Resistance
- Enzyme Inhibitors
- Fibroblasts