Article
Genetic correction of cultured T cells from an adenosine deaminase-deficient patient: characteristics of non-transduced and transduced T cells.
European journal of immunology - 1 Jan 1992
Braakman E, Van Beusechem V W, Van Krimpen B A, Fischer A, Bolhuis R L, Valerio D
Abstract excerpt
T lymphocytes derived from peripheral blood of a patient with adenosine deaminase (ADA) deficiency were expanded in vitro. The human ADA (hADA) gene was introduced into these replicating ADA- T cells with the use of an amphotropic recombinant retrovirus carrying the hADA gene. Subsequently, infected T cells were selected on the basis of their ADA expression, by exposure to a combination of the toxic agent...
Topics
- Adenosine Deaminase
- CD4 Antigens
- CD8 Antigens
- Cells, Cultured
- Genetic Therapy
- Humans
- Infant
- Phenotype
- T-Lymphocytes
- Transduction, Genetic