Mutation typing using electrophoresis and gel-immobilized Acrydite probes.

doi:10.2144/98253pf01

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Article

Mutation typing using electrophoresis and gel-immobilized Acrydite probes.

BioTechniques - 1 Sept 1998

Kenney M, Ray S, Boles T C

Abstract excerpt

A new electrophoresis technology for hybridization-based sequence detection and mutation typing is described. Intrinsic to this approach is copolymerization of specially modified oligonucleotide probes directly into polyacrylamide gels. Electrophoresis of single-stranded samples through gels cont...

Topics

DNA Mutational Analysis
DNA Probes
DNA, Single-Stranded
DNA, Viral
Electrophoresis, Polyacrylamide Gel
Genes, Viral
HIV
Humans
Mutation
Nucleic Acid Hybridization
Organophosphorus Compounds
Polymorphism, Genetic
RNA-Directed DNA Polymerase
Sensitivity and Specificity

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