Article
Mutation typing using electrophoresis and gel-immobilized Acrydite probes.
BioTechniques - 1 Sept 1998
Kenney M, Ray S, Boles T C
Abstract excerpt
A new electrophoresis technology for hybridization-based sequence detection and mutation typing is described. Intrinsic to this approach is copolymerization of specially modified oligonucleotide probes directly into polyacrylamide gels. Electrophoresis of single-stranded samples through gels containing specific immobilized probes results in hybridization-mediated capture of complementary targets. By increasing...
Read the complete abstract on PubMedTopics
- DNA Mutational Analysis
- DNA Probes
- DNA, Single-Stranded
- DNA, Viral
- Electrophoresis, Polyacrylamide Gel
- Genes, Viral
- HIV