Article
Direct fluorescence analysis of genetic polymorphisms by hybridization with oligonucleotide arrays on glass supports.
Nucleic acids research - 11 Dec 1994
Guo Z, Guilfoyle R A, Thiel A J, Wang R, Smith L M
Abstract excerpt
A simple and rapid method for the analysis of genetic polymorphisms has been developed using allele-specific oligonucleotide arrays bound to glass supports. Allele-specific oligonucleotides are covalently immobilized on glass slides in arrays of 3 mm spots. Genomic DNA is amplified by PCR using o...
Topics
- Alleles
- Amino Acid Sequence
- Base Sequence
- Cross-Linking Reagents
- Exons
- Fluorescein
- Fluoresceins
- Glass
- Humans
- Image Processing, Computer-Assisted
- Molecular Probe Techniques
- Molecular Sequence Data
- Monophenol Monooxygenase
- Nucleic Acid Hybridization
- Oligonucleotide Probes