Article
Detection of specific alleles by using allele-specific primer extension followed by capture on solid support.
Genetic analysis, techniques and applications - 1 Aug 1992
Ugozzoli L, Wahlqvist J M, Ehsani A, Kaplan B E, Wallace R B
Abstract excerpt
This article describes a method for determining whether a particular nucleic acid sequence is present in a sample and for discriminating between any two nucleic acid sequences if such sequences differ only by a single nucleotide. The method entails extension of a novel two-component primer on templates that may or may not include a target nucleic acid sequence. The 3' portion of the primer is complementary to a...
Topics
- Alleles
- Base Sequence
- DNA
- Electrophoresis, Polyacrylamide Gel
- Humans
- Molecular Sequence Data
- Monophenol Monooxygenase
- Polymorphism, Genetic