Article
Genetic analysis using polymerase chain reaction-amplified DNA and immobilized oligonucleotide probes: reverse dot-blot typing.
Methods in enzymology - 1 Jan 1993
Kawasaki E, Saiki R, Erlich H
Abstract excerpt
The reverse dot-blot method is a simple and rapid diagnostic procedure that allows screening of sample for a variety of mutations/polymorphisms in a single hybridization reaction. Several methods of immobilizing the oligonucleotide probes are discussed. The reverse dot-blot method has several uni...
Topics
- Amino Acid Sequence
- Base Sequence
- Codon
- DNA, Neoplasm
- Genes, ras
- Humans
- Immunoblotting
- Molecular Sequence Data
- Mutation
- Nucleic Acid Hybridization
- Oligonucleotide Probes
- Polymerase Chain Reaction
- Polymorphism, Genetic
- Tumor Cells, Cultured