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Article

Evidence for a symmetrical requirement for Rab5-GTP in in vitro endosome-endosome fusion.

The Journal of biological chemistry - 2 Oct 1998

Barbieri M A, Hoffenberg S, Roberts R, Mukhopadhyay A, Pomrehn A, Dickey B F, Stahl P D

Abstract excerpt

Early endosome fusion, which has been extensively characterized using an in vitro reconstitution assay, is Rab5-dependent. To examine the requirement for Rab5 on both fusion partners, we prepared cytosol and endosomes depleted of Rab5. Unlike control cytosol, Rab5-depleted cytosol was only marginally active in the in vitro endosome fusion. However, fusion could be restored by the addition of wild-type Rab5 or...

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Identifiers

PMID: 9748259
DOI: 10.1074/jbc.273.40.25850

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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Evidence for a symmetrical requirement for Rab5-GTP in in vitro endosome-endosome fusion.PMID 9748259Read ↗

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