Article
Evidence for a symmetrical requirement for Rab5-GTP in in vitro endosome-endosome fusion.
The Journal of biological chemistry - 2 Oct 1998
Barbieri M A, Hoffenberg S, Roberts R, Mukhopadhyay A, Pomrehn A, Dickey B F, Stahl P D
Abstract excerpt
Early endosome fusion, which has been extensively characterized using an in vitro reconstitution assay, is Rab5-dependent. To examine the requirement for Rab5 on both fusion partners, we prepared cytosol and endosomes depleted of Rab5. Unlike control cytosol, Rab5-depleted cytosol was only margin...
Topics
- Animals
- Endosomes
- GTP Phosphohydrolases
- GTP-Binding Proteins
- Green Fluorescent Proteins
- Guanosine Triphosphate
- Luminescent Proteins
- Macrophages
- Membrane Fusion
- Mice
- Microscopy, Fluorescence
- Mutation
- Protein Binding
- Ribonucleotides
- rab5 GTP-Binding Proteins