Article
The CHO XRCC1 mutant, EM9, deficient in DNA ligase III activity, exhibits hypersensitivity to camptothecin independent of DNA replication.
Mutation research - 7 Aug 1998
Barrows L R, Holden J A, Anderson M, D'Arpa P
Abstract excerpt
We have analyzed the X-ray-sensitive CHO mutant cell line EM9 for sensitivity to the topoisomerase I inhibitor comptothecin. These cells exhibit defective repair of single strand DNA breaks. Recently, EM9 were complemented the DNA ligase III interactive protein, XRCC1. Defective XRCC1 apparently...
Topics
- Animals
- Aphidicolin
- Blotting, Western
- CHO Cells
- Camptothecin
- Cell Survival
- Cricetinae
- DNA Ligase ATP
- DNA Ligases
- DNA Replication
- DNA Topoisomerases, Type I
- DNA-Binding Proteins
- Gene Deletion
- Mutation
- Poly-ADP-Ribose Binding Proteins
- S Phase
- Topoisomerase I Inhibitors
- X-ray Repair Cross Complementing Protein 1