Article
Transmembrane protein insertion orientation in yeast depends on the charge difference across transmembrane segments, their total hydrophobicity, and its distribution.
The Journal of biological chemistry - 18 Sept 1998
Harley C A, Holt J A, Turner R, Tipper D J
Abstract excerpt
The determinants of transmembrane protein insertion orientation at the endoplasmic reticulum have been investigated in Saccharomyces cerevisiae using variants of a Type III (naturally exofacial N terminus (Nexo)) transmembrane fusion protein derived from the N terminus of Ste2p, the alpha-factor...
Topics
- Amino Acid Sequence
- Base Sequence
- DNA Primers
- Endoplasmic Reticulum
- Genetic Variation
- Intracellular Membranes
- Membrane Fusion
- Molecular Sequence Data
- Polymerase Chain Reaction
- Protein Conformation
- Receptors, Mating Factor
- Receptors, Peptide
- Recombinant Fusion Proteins
- Saccharomyces cerevisiae
- Static Electricity
- Transcription Factors