Article
The role of the N region in signal sequence and signal-anchor function.
The Journal of biological chemistry - 15 Apr 1992
Andrews D W, Young J C, Mirels L F, Czarnota G J
Abstract excerpt
To determine the role of sequences other than the hydrophobic core in mediating signal sequence function, we examined the behavior of fusion proteins and deletion mutants in cell-free systems. We demonstrate that neither the N nor the C region of the preprolactin signal sequence is necessary for translocation. However, insertion of sequences with either a net charge of +2.5 or -6.0 between the N region and the...
Topics
- Amino Acid Sequence
- Animals
- Cell-Free System
- Electrophoresis, Polyacrylamide Gel
- Immunoglobulin M
- Membrane Proteins
- Molecular Sequence Data
- Mutation
- Pan troglodytes
- Plasmids
- Prolactin