Article
Genetic analysis of poliovirus protein 3A: characterization of a non-cytopathic mutant virus defective in killing Vero cells.
The Journal of general virology - 1 Aug 1998
Lama J, Sanz M A, Carrasco L
Abstract excerpt
A mutational and genetic analysis of the poliovirus protein 3A has led to the identification of a single amino acid mutant virus with a restrictive phenotype to form plaques in Vero cells. This mutant (I46T 3A) can be grown and amplified in HeLa cells, where virus replication takes place at wild-...
Topics
- Amino Acid Substitution
- Animals
- Cell Death
- Cell Extracts
- Chlorocebus aethiops
- Cytopathogenic Effect, Viral
- Defective Viruses
- HeLa Cells
- Humans
- Isoleucine
- Phenotype
- Point Mutation
- Poliovirus
- Protein Processing, Post-Translational
- Proteins
- Threonine
- Vero Cells
- Viral Core Proteins