Article
Isolation and characterization of pokeweed antiviral protein mutations in Saccharomyces cerevisiae: identification of residues important for toxicity.
Proceedings of the National Academy of Sciences of the United States of America - 29 Aug 1995
Hur Y, Hwang D J, Zoubenko O, Coetzer C, Uckun F M, Tumer N E
Abstract excerpt
Pokeweed antiviral protein (PAP), a 29-kDa protein isolated from Phytolacca americana inhibits translation by catalytically removing a specific adenine residue from the 28S rRNA of eukaryotic ribosomes. PAP has potent antiviral activity against many plant and animal viruses, including human immunodeficiency virus. We describe here development of a positive selection system to isolate PAP mutants with reduced...
Topics
- Cloning, Molecular
- DNA, Complementary
- Mutation
- N-Glycosyl Hydrolases
- Plant Proteins
- Plasmids
- Point Mutation
- Protein Processing, Post-Translational
- Ribosome Inactivating Proteins, Type 1
- Saccharomyces cerevisiae