Article
Effect of target gene CpG content on spontaneous mutation in299 transgenic mice.
Mutation research - 25 May 1998
Skopek T, Marino D, Kort K, Miller J, Trumbauer M, Gopal S, Chen H
Abstract excerpt
Transgenic mutation assays utilizing bacterial target genes display a high frequency of spontaneous mutation at CpG sequences. This is believed to result from the fact that: (1) the prokaryotic genes currently being used as transgenic mutation targets have a high CpG content and (2) these sequences are methylated by mammalian cells to produce 5-methylcytosine (5MC), a known promutagenic base. To study the effect...
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