Article
Effect of target gene CpG content on spontaneous mutation in299 transgenic mice.
Mutation research - 25 May 1998
Skopek T, Marino D, Kort K, Miller J, Trumbauer M, Gopal S, Chen H
Abstract excerpt
Transgenic mutation assays utilizing bacterial target genes display a high frequency of spontaneous mutation at CpG sequences. This is believed to result from the fact that: (1) the prokaryotic genes currently being used as transgenic mutation targets have a high CpG content and (2) these sequenc...
Topics
- Animals
- Bacterial Proteins
- Base Sequence
- CpG Islands
- DNA Mutational Analysis
- Escherichia coli Proteins
- Gene Targeting
- Genes, Bacterial
- Genetic Vectors
- Lac Repressors
- Mice
- Mice, Transgenic
- Molecular Sequence Data
- Mutation
- Point Mutation
- Recombinant Fusion Proteins
- Repressor Proteins
- Transgenes