Article
Physical mapping of a collection of Mael-generating amber mutations in the beta gene of Escherichia coli RNA polymerase and the functional effect of internal deletions constructed through their manipulation.
Genes and function - 1 Apr 1997
Buyukuslu N, Trigwell S M, Lim P P, Fujita N, Ishihama A, Ralphs N T, Glass R E
Abstract excerpt
We report the fine mapping of 55 of our 95 amber mutations in the beta gene of Escherichia coli RNA polymerase by virtue of the unique MaeI restriction sites created by this subset of nonsense mutations (i.e. CTAG, where the amber codon is underlined). [The full data are reported in Supplementary Publication SUP 50181 (12 pages), which has been deposited at the British Library Document Supply Centre, Boston Spa,...
Topics
- Amino Acid Sequence
- Cloning, Molecular
- DNA-Directed RNA Polymerases
- Deoxyribonucleases, Type II Site-Specific
- Escherichia coli
- Gene Deletion
- Mutation
- Plant Proteins
- Plasmids
- Restriction Mapping