Article
In vivo cloning of a carboxy-terminal rpoB allele which confers altered transcriptional properties.
Folia microbiologica - 1 Jan 1995
Rowland G C, Lim P P, Glass R E
Abstract excerpt
A 3'-terminal mutation of the gene encoding the beta subunit of Escherichia coli RNA polymerase was isolated using an in vivo polA(Ts) technique. Cloning of the allele was monitored by virtue of the fact that the deletion delta(rpoB)1570-1 resulted in an altered-size restriction fragment. DNA sequencing confirmed the predicted nature and location of the mutation: delta(rpoB)1570-1 involved an in-frame deletion of...
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