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Article

Insertional mutagenesis of a plasmid-borne Escherichia coli rpoB gene reveals alterations that inhibit beta-subunit assembly into RNA polymerase.

Journal of bacteriology - 1 Jun 1990

Landick R, Colwell A, Stewart J

Abstract excerpt

A plasmid was constructed that overproduces the Escherichia coli RNA polymerase beta subunit from a lac promoter-rpoB fusion. The overproduced, plasmid-encoded beta subunit assembled into functional RNA polymerase that supplied greater than 90% of the transcriptional capacity of the cells. Excess beta subunit segregated into insoluble inclusion bodies and was not deleterious to cell growth. By insertion of a XhoI...

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Identifiers

PMID: 2160927
DOI: 10.1128/jb.172.6.2844-2854.1990
PMCID: PMC209080

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