Article
Insertional mutagenesis of a plasmid-borne Escherichia coli rpoB gene reveals alterations that inhibit beta-subunit assembly into RNA polymerase.
Journal of bacteriology - 1 Jun 1990
Landick R, Colwell A, Stewart J
Abstract excerpt
A plasmid was constructed that overproduces the Escherichia coli RNA polymerase beta subunit from a lac promoter-rpoB fusion. The overproduced, plasmid-encoded beta subunit assembled into functional RNA polymerase that supplied greater than 90% of the transcriptional capacity of the cells. Excess...
Topics
- Base Sequence
- DNA Transposable Elements
- DNA-Directed RNA Polymerases
- Escherichia coli
- Genes, Bacterial
- Inclusion Bodies
- Molecular Sequence Data
- Mutation
- Plasmids
- Protein Conformation
- Rifampin
- Structure-Activity Relationship