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Article

Insights into RNA polymerase catalysis and adaptive evolution gained from mutational analysis of a locus conferring rifampicin resistance.

Nucleic acids research - 2 Nov 2017

Yurieva Olga, Nikiforov Vadim, Nikiforov Vadim, O'Donnell Michael, Mustaev Arkady

Abstract excerpt

S531 of Escherichia coli RNA polymerase (RNAP) β subunit is a part of RNA binding domain in transcription complex. While highly conserved, S531 is not involved in interactions within the transcription complex as suggested by X-ray analysis. To understand the basis for S531 conservation we performed systematic mutagenesis of this residue. We find that the most of the mutations significantly decreased...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Share to DNA-Directed RNA Polymerases

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Identifiers

PMID: 29036608
DOI: 10.1093/nar/gkx813
PMCID: PMC5737076

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