Article
Genotyping of human apolipoprotein E alleles by the new qualitative, microplate-based CASSI-detection assay.
BioTechniques - 1 Jul 1998
Köhler T, Rost A K, Purschwitz K, Vondran S, Remke H, Wagner O, Richter V
Abstract excerpt
A new qualitative PCR product detection assay called competitive amplified single mutation detection by selective probe hybridization immunoassay (CASSI) was developed for genotyping the most common apolipoprotein E (apoE) polymorphisms. Single target DNA strands immobilized using biotin on streptavidin-coated microplates were hybridized in separate wells with two distinct, 5'-fluorescein isothiocyanate...
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