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Article

Detection of apoB-100 R3500Q mutation by competitive allele-specific polymerase chain reaction.

Journal of clinical laboratory analysis - 1 Jan 2001

Horvath A D, Kirov S A, Karaulanov E E, Ganev V S

Abstract excerpt

The apolipoprotein B-100 mutation R3500Q is one of the most common inherited defects causing abnormality of the lipid metabolism. We describe a one-step, single-tube PCR technique for detection of the mutation based on competition between allele-specific primers. Three oligonucleotides are used: two allele-specific primers differing in their 3' nucleotide (for the wild-type and the mutant allele) together with a...

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Identifiers

PMID: 11574954
DOI: 10.1002/jcla.1037
PMCID: PMC6807702

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Source receipt: pubmed, retrieved 7 Sept 2026.

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