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Article

Apolipoprotein E genotyping method by real time PCR, a fast and cost-effective alternative to the TaqMan and FRET assays.

Journal of neuroscience methods - 15 Oct 2009

Calero Olga, Hortigüela Rafael, Bullido María J, Calero Miguel

Abstract excerpt

The apolipoprotein E gene (APOE) polymorphism genotyping has an allegedly important predictive value for coronary heart disorders and Alzheimer's disease. We developed a simple, fast, cost-effective and suited for high-throughput protocol for determining APOE genotypes by Real Time PCR monitored by SYBR Green. The method is based on differential amplification by allele-specific primers. These primers have...

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Source

Identifiers

PMID: 19583979
DOI: 10.1016/j.jneumeth.2009.06.033

Genes

APOE

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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