Article
Single nucleotide polymorphism typing on DNA array with hydrophobic surface fabricated by plasma-polymerization technique.
Biosensors & bioelectronics - 15 Sept 2004
Miyachi Hirotaka, Ikebukuro Kazunori, Yano Kazuyoshi, Aburatani Hiroyuki, Karube Isao
Abstract excerpt
A DNA array has been fabricated on glass substrates, which enables high-throughput analysis of single-base mismatches. In this work, microfabrication-compatible plasma-polymerization (PP) method was used for immobilizing probe DNAs to study the hybridization behavior by changing surface properties. The immobilization matrix consisting of 35 A of PP layer, applied additionally on the streptavidin absorbed...
Topics
- Apolipoproteins E
- Coated Materials, Biocompatible
- DNA Mutational Analysis
- DNA Probes
- Equipment Design
- Equipment Failure Analysis
- Gene Expression Profiling
- Hot Temperature
- Hydrophobic and Hydrophilic Interactions
- Manufactured Materials
- Oligonucleotide Array Sequence Analysis
- Polymerase Chain Reaction