Article
Estimation of single-nucleotide polymorphism allele frequency by alternately binding probe competitive polymerase chain reaction.
Analytica chimica acta - 11 Feb 2008
Noda Naohiro, Tani Hidenori, Morita Nao, Kurata Shinya, Nakamura Kazunori, Kanagawa Takahiro, Tsuneda Satoshi, Sekiguchi Yuji
Abstract excerpt
Estimation of single-nucleotide polymorphism (SNP) allele frequency in pooled DNA samples is a promising approach to clarify the relationships between SNPs and diseases. Here, we present a simple, accurate, and cost-effective method for estimating SNP allele frequency, called alternately binding probe (ABProbe) competitive polymerase chain reaction (ABC-PCR) that entails no expensive devices for real-time...
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