Article
A surface invasive cleavage assay for highly parallel SNP analysis.
Human mutation - 1 Apr 2002
Lu Manchun, Shortreed Michael R, Hall Jeff G, Wang Liman, Berggren Travis, Stevens Priscilla Wilkins, Kelso David M, Lyamichev Victor, Neri Bruce, Smith Lloyd M
Abstract excerpt
The structure-specific invasive cleavage of single-stranded DNA by 5' nucleases is a useful means for sensitive detection of single-nucleotide polymorphisms or SNPs. The solution-phase invasive cleavage reaction has sufficient sensitivity for direct detection of as few as 600 target molecules with no prior target amplification. One approach to the parallelization of SNP analysis is to adapt the invasive cleavage...
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